Part:BBa_K2206001:Design

Toehold switch for hsa-miR-27b-3p

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We ensured that there are no stop codons between the start codon and the protein coding sequence.

The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing at most.

The distance between the start codon and the protein coding region has to be a multiple of three.

For more detail visit: http://2017.igem.org/Team:CLSB-UK/Design

Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.